Cre-Expressing Human Primary Bronchial Fibroblasts

Catalog Number: BVX-1988Cre Product Format: Frozen Vial Cell Number: > 5x10[5] cells/vial Passage:1(P1) Storage: Liquid Nitrogen, Vapor Phase Intended Use: Research use only (RUO) CELL CHARACTERISTICS Origin: These cells are derived from human primary bronchial tissue, carefully harvested from biobanked samples to ensure high quality and relevance to human physiology. - Genetic Modification: Engineered to express Cre recombinase, enabling researchers to perform precise conditional gene knockout experiments through the Cre-loxP system. This feature facilitates the temporal and spatial control of gene deletion, crucial for understanding gene function in vivo. - Morphology: Exhibiting a fibroblast-like morphology, they are characterized by elongated, spindle-shaped cells that adhere firmly to tissue culture plastic, allowing for efficient proliferation. - Growth Conditions: Cells thrive in a humidified incubator maintained at 37°C with 5% CO2, mimicking physiological conditions for optimal growth. - Media Recommendations: Cultivation is best achieved in fibroblast growth medium supplemented with 10% fetal bovine serum (FBS) and additional growth factors tailored to promote fibroblast viability and function.